GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
HVJ (hemagglutinating virus of Japan) Envelope VECTOR KIT is a tool for transfection of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion.
The HVJ envelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope.
GenomONE-Neo EX provides ready-to-use kits containing the HVJ envelope and auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer)
Application
[1] siRNA transfection
in vitro
Primary T cell (Human peripheral blood)
Primary cardiac myocyte (Rat cardiac myocyte)
Differentiated C2C12 (Mouse myoblast)
monkey ES cells
Min6 (Mouse pancreatic β cell)
U937 (Human myelomonocytic cell)
in vivo
ntradermally transplanted human cervix cancer /HeLa (SCID mouse)
Submandibular gland (rat)
[2] Protein delivery
in vitro
Primary macrophage (C3H mouse peritoneal resident)
Swiss 3T3 cell (Mouse embryonic fibroblasts)
in vivo
Nucleus tractus solitarius (Rat brain)
[3] Oligo DNA transfection
in vitro
Primary HDMECs (Human dermal microvascular endothelial cell)
Primary CD34+ cell (Human blood)
in vivo
Uterus (Mouse)
Skin, ear lobe (Mouse)
Lung (Mouse)
[4] Plasmid DNA transfection
in vivo
Uterus(Mouse)
Palatal periodontal tissue (Rat)
Myocardium (Rat heart)
Lung (neonatal porcine)
Subcutaneously transplanted human colon cancer /LoVo (nude mouse)